Pre-packaged AAV: All the following pre-packaged AAVs were pre-made with serotype 1**, 2, 5, 6, 8** and 9** as controls. All the pre-packaged rAAVs are accurately titered via qPCR, packed in 200* or 100* μl and in ready-to-use format.
* While we guarantee delivery of total >2E+11 GC per vial, the actual volume may vary per the actual rAAV titer determined via qPCR. If rAAV titer is >1E+13 GC/ml, the volume will be 20 ul. ** Some terms may apply to purchase of certain serotype of pre-packaged AAVs.
Product Category: recombinant AAV
Description: AAV9-CAG-GFP is the serotype 9 AAV which express eGFP under the control CAG (also known as CBA) promoter which is a combination of the Cytomegalovirus (CMV) early enhancer element and chicken beta-actin promoter for high levels of gene expression in mammalian expression vectors. Used as a control.
Titer: 1×1012 ~ 1×1013 GC/ml
Size: 200 ul
Storage Buffer: PBS with 5% Glycerol
Infection Protocol:
Product Category: Recombinant AAV, AAV9-CMV-LacZ
Description:
AAV9-LacZ?is the seroptype 9 rAAV which expresses β-galactosidase (LacZ) under CMV promoter. LacZ is frequently ?used as reporter gene. It can be easily located with a LacZ stain using the artificial substrate X-gal, which turns blue when it is cleaved by β-galactosidase.
Titer: 1×1012 ~ 1×1013 GC/ml
Size: 200 ul
Storage Buffer: PBS with 5% Glycerol
Infection Protocol:
Product Category: recombinant AAV
Description: AAV9-ChR2-YFP is the serotype 9 AAV which express Channelrhodopsin-2 under CAG promoter with YFP tag. Channelrhodopsins are a subfamily of opsin proteins that function as light-gated ion channels and very useful for many bioengineering and neuroscience applications such as photostimulation of neurons for probing of neural circuits. Using? a Yellow Fluorescent Protein (YFP) tagged ChR2, light-stimulated axons and synapses can be identified in...
Product Category: recombinant AAV
Description: AAV9-Synapsin-GFP is the serotype 9 AAV which express eGFP under the Synapsin promoter which restricts transgene expression only in neurons.
Titer: 1×1012 ~ 1×1013 GC/ml
Size: 100 ul
Storage Buffer: PBS with 5% Glycerol
Infection Protocol:
Product Category: recombinant AAV, AAV9-ChIEF-tdTOMATO
Description:
AAV9-ChIEF-tdTOMATO the serotype 9 rAAV which expresses a hybrid of Channelrhodopsin-1/2 under CAG promoter with tdTOMATO tag.
ChIEF is a hybrid of channelrhodopsin 1 and 2 with additional L170I mutation (ChR1 numbering) or at L131I (ChR2-numbering), leading to large currents in oocytes and HEK-cells and almost wild type like open state life time. ChIEF function as light-gated ion channels and very useful for many bioenginee...
Product Category: recombinant AAV, AAV9-CMV-mRFP
Description:
AAV9-mRFP is the serotype 9 rAAV which expresses mRFP under the CMV promoter. AAV9-mRFP express a monomeric red fluorescent protein mutant from Discosoma (DsRed) , overcome the tetramerization which is toxic to cells. mRFP can be used as a reporter in target cells. Used as a control.
Titer: 1×1012 ~ 1×1013 GC/ml
Size: 200 ul
Storage Buffer: PBS with 5% Glycerol
Infection Protocol:
Product Name: AAV Serotypes Testing Kit
Product Category: recombinant AAV
Description: This kits contains AAV1-CMV-GFP, AAV2-CMV-GFP, AAV5-CMV-GFP, AAV6-CMV-GFP, AAV8-CMV-GFP and AAV9-CMV-GFP viral stocks. It can be used for testing the infection efficiency on different cell types to see which serotype works best.
Titer: 1E+12~E+13 GC/ml
Size: 200 μl of each of the viral stock
Storage Buffer: PBS
Infection Protocol: