Pre-packaged AAV: All the following pre-packaged AAVs were pre-made with serotype 1**, 2, 5, 6, 8** and 9** as controls. All the pre-packaged rAAVs are accurately titered via qPCR, packed in 200* or 100* μl and in ready-to-use format.
* While we guarantee delivery of total >2E+11 GC per vial, the actual volume may vary per the actual rAAV titer determined via qPCR. If rAAV titer is >1E+13 GC/ml, the volume will be 20 ul. ** Some terms may apply to purchase of certain serotype of pre-packaged AAVs.
Product Category: recombinant AAV, AAV1-CMV-mRFP
Description:
AAV1-mRFP is the serotype 2/1 (with Capsid from AAV1 and ITR from AAV2) which expresses mRFP under the CMV promoter. AAV1-mRFP express a monomeric red fluorescent protein mutant from Discosoma (DsRed) , overcome the tetramerization which is toxic to cells. mRFP can be used as a reporter in target cells. Used as a control.
Titer: 1×1012 ~ 1×1013 GC/ml
Size: 200 ul
Storage Buffer: PBS with 5% Glycerol
Infection Protoc...
Product Category: Recombinant AAV, AAV2-CMV-Null
Description:
AAV2-Null is the serotype 2 AAV with CMV promoter with no insert gene. Used as a control.
Titer: 1×1012 ~ 1×1013 GC/ml
Size: 200 ul
Storage Buffer: PBS with 5% Glycerol
Infection Protocol:
Product Category: recombinant AAV, AAV2-CMV-GFP
Description:
AAV2-GFP is the serotype 2 AAV which express eGFP under the CMV promoter. Used as a control
Titer: 1×1012 ~ 1×1013 GC/ml
Size: 200 ul
Storage Buffer: PBS with 5% Glycerol
Infection Protocol:
Product Category: recombinant AAV, AAV2-CMV-Cre
Description: AAV2-Cre is the serotype 2 AAV which express Cre recombinase under the control CMV promoter. This product used in the Cre-lox system as a genetic tool to generate site-specific recombination of DNA between loxP sites in cultured cells and animal experiments.
Titer: 1×1012 ~ 1×1013 GC/ml
Size: 200 ul
Storage Buffer: PBS with 5% Glycerol
Infection Protocol:
Product Category: recombinant AAV, AAV2-CMV-Cre-GFP
Description: AAV2-Cre-GFP is the serotype 2 AAV which express Cre recombinase and eGFP under the control CMV promoter in two separate expression cassette. This product used in the Cre-lox system as a genetic tool to generate site-specific recombination of DNA between loxP sites in cultured cells and animal experiments.
Titer: 1×1012 ~ 1×1013 GC/ml
Size: 200 ul
Storage Buffer: PBS with 5% Glycerol
Infection Protocol:
Product Category: recombinant AAV, AAV2-U6-shRNA-GFP
Description: AAV2-U6-shRNA-GFP is the serotype 2 AAV which expresses scramble shRNA under U6 promoter with co-expression of GFP as reporter under CMV promoter. This product used in the gene knockdown experiments as a control in cultured cells and animal experiments.
Titer: 1×1012 ~ 1×1013 GC/ml
Size: 200 ul
Storage Buffer: PBS with 5% Glycerol
Infection Protocol:
Product Category: recombinant AAV
Description: AAV2-CAG-GFP is the serotype 2 AAV which express eGFP under the control CAG (also known as CBA) promoter which is a combination of the cytomegalovirus (CMV) early enhancer element and chicken beta-actin promoter for high levels of gene expression in mammalian expression vectors. Used as a control.
Titer: 1×1012 ~ 1×1013 GC/ml
Size: 200 ul
Storage Buffer: PBS with 5% Glycerol
Infection Protocol:
Product Category: Recombinant AAV, AAV2-CMV-LacZ
Description:
AAV2-LacZ?is the seroptype 2 rAAV which expresses β-galactosidase (LacZ) under CMV promoter. LacZ is frequently ?used as reporter gene. It can be easily located with a LacZ stain using the artificial substrate X-gal, which turns blue when it is cleaved by β-galactosidase.
Titer: 1×1012 ~ 1×1013 GC/ml
Size: 200 ul
Storage Buffer: PBS with 5% Glycerol
Infection Protocol:
Product Category: recombinant AAV, AAV2-CMV-mRFP
Description:
AAV2-mRFP is the serotype 2 rAAV which expresses mRFP under the CMV promoter. AAV2-mRFP express a monomeric red fluorescent protein mutant from Discosoma (DsRed) , overcome the tetramerization which is toxic to cells. mRFP can be used as a reporter in target cells. Used as a control.
Titer: 1×1012 ~ 1×1013 GC/ml
Size: 200 ul
Storage Buffer: PBS with 5% Glycerol
Infection Protocol:
Product Category: recombinant AAV
Description: AAV2-ChR2-YFP is the serotype 2 AAV which express Channelrhodopsin-2 under CAG promoter with YFP tag. Channelrhodopsins are a subfamily of opsin proteins that function as light-gated ion channels and very useful for many bioengineering and neuroscience applications such as photostimulation of neurons for probing of neural circuits. Using a Yellow Fluorescent Protein (YFP) tagged ChR2, light-stimulated axons and synapses can be identified in i...