GenJet? In Vitro DNA Tranfection Reagent for Hela Cells
￥ 1,560.00
購買(mǎi)數量：
目錄號: SL100489-HELA
包裝: 1.0 ml
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Description
GenJet? DNA In Vitro
Tranfection Reagent for Hela is pre-optimized for Hela cell transfection.
Refer to the following optimal transfection conditions for maximal transfection
efficiency on Hela cells. GenJet? reagent, 1.0 ml, is sufficient for 600 to
1200 transfections in 24 well plates or 300 to 600 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
~80%
Cell culture conditions
DMEM with 4.5 g/L glucose, 10% FBS
GenJet? (μl) : DNA (μg) Ratio
3:1
Diluent for DNA and Transfection Reagent
Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet?/DNA Complex
15 minutes at RT
Presence of Serum/Antibiotics during Transfection
Yes
Change Medium 5 Hours After Transfection
Yes
Maximal Efficiency
48 hours
Transfection Results:
Reporter Gene
EGFP
Plasmid
pEGFP-N3 (CMV promoter)
Efficiency (GFP %)
88%
Storage Condition Store at 4 °C. If stored properly, the product is stable for 12 months or longer A Picture Showing Transfection Efficiency of GenJet? Reagent on Hela Cells
GenJet? reagent is optimized for Hela cell (ATCC # CCL-2.2). Hela cells
were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by
GenJet?. The efficiency was checked 48 hours post transfection Data Sheet
