Description
GenJet? DNA In Vitro
Tranfection Reagent for U2OS cells is pre-optimized for transfecting U2OS cells.
The U2OS cell line, originally known as the 2T line, was cultivated from the
bone tissue of a fifteen-year-old human female suffering from osteosarcoma.
Established in 1964, the original cells were taken from a moderately
differentiated sarcoma of the tibia. U2 OS cells exhibit epithelial
adherent morphology and viruses were not detected in the line during
co-cultivation with WI-38 cells or in CF tests against SV40, RSV, or
adenoviruses. Cells are positive for insulin-like growth factor I (IGF-I) and
insulin-like growth factor II (IGF II) receptors and express a number of
antigens, including blood type A, Rh+, HLA A2, Aw30, B12, Bw35, and B40(+/-).
Refer to the following optimal transfection conditions for maximal transfection
efficiency on U2 OS cells. GenJet? reagent, 1.0 ml, is sufficient for 300 to
600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
|
60~70% |
| Cell culture conditions |
DMEM with 4.5 g/L glucose, 10% FBS |
| GenJet? (μl) : DNA (μg) Ratio |
3:1 |
Diluent for DNA and Transfection Reagent
|
Serum-free DMEM with 4.5 g/L glucose
|
| Incubation Time to Form GenJet?/DNA Complex |
15 minutes at RT |
| Presence of Serum/Antibiotics during Transfection |
Yes |
| Change Medium 5 Hours After Transfection |
No |
| Maximal Efficiency |
48 hours |
Transfection Results:
Reporter Gene
|
EGFP |
Plasmid
|
pEGFP-N3 (CMV promoter)
|
| Efficiency (GFP %) |
95% |
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A Picture Showing Transfection Efficiency of GenJet? Reagent on U2 OS Cells
GenJet? reagent is optimized for
U2OS. U2OS cells
were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by
GenJet?. The efficiency was checked 48 hours post transfection
Data Sheet 
