GenJet? In Vitro DNA Tranfection Reagent for CHO Cells
￥ 1,560.00
購買(mǎi)數量：
目錄號: SL100489-CHO
包裝: 1.0 ml
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Description
GenJet? DNA In Vitro
Tranfection Reagent for CHO is pre-optimized
for transfecting Chinese Hamster Ovary (CHO) cells.
Refer to the following optimal transfection conditions for maximal transfection
efficiency on CHO cells. GenJet? reagent, 1.0 ml, is sufficient for 300 to
600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
~90%
Cell culture conditions
DMEM with 4.5 g/L glucose, 10% FBS
GenJet? (μl) : DNA (μg) Ratio
3:1
Diluent for DNA and Transfection Reagent
Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet?/DNA Complex
15 minutes at RT
Presence of Serum/Antibiotics during Transfection
Yes
Change Medium After Transfection
Yes, 16~24 Hours After Transfection
Maximal Efficiency
48 hours
Transfection Results:
Reporter Gene
EGFP
Plasmid
pEGFP-N3 (CMV promoter)
Efficiency (GFP %)
90%
Storage Condition Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A Picture Showing
Transfection Efficiency of GenJet? Reagent on CHO Cells. CHO cells
were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by
GenJet?. The efficiency was checked 24 hours post transfection
Data Sheet To request a free trial sample, please Create An Account with us to enter your shipping address and email us at order@signagen.com
