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GenJet? In Vitro DNA Tranfection Reagent for Jurkat Cell

￥ 2,680.00

購買(mǎi)數量：

  • 目錄號: SL100489-JURKAT
  • 包裝: 1.0 ml

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Description

GenJet? DNA In Vitro Tranfection Reagent for Jurkat cell is pre-optimized for transfecting Jurkat cells. Refer to the following optimal transfection conditions for maximal transfection efficiency on Jurkat cells. GenJet? reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:

Confluence on the day of transfection
 ~70%
Cell culture conditions PRMI with 10% FBS
GenJet? (μl) : DNA (μg) Ratio 3:1
Diluent for DNA and Transfection Reagent
 1xGenJet? Transfection Buffer
Incubation Time to Form GenJet?/DNA Complex 15 minutes at RT
Presence of Serum/Antibiotics during Transfection Yes
Change Medium After Transfection No
Maximal Efficiency 48 hours

Transfection Results:

Reporter Gene
 EGFP
Plasmid
 pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 80%

Storage Condition
 Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet & Protocol




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