GenJet? In Vitro DNA Tranfection Reagent for Rin Related Cells
￥ 1,560.00
購買(mǎi)數量：
目錄號: SL100489-RIN
包裝: 1.0 ml
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Description
GenJet? DNA In Vitro Tranfection Reagent for Rin is pre-optimized znd conditioned for transfecting Rin and related cells including Rin-14B, Rin-m5F, Rin-m and Rin-5F. Refer to the following optimal transfection conditions for maximal transfection efficiency on Rin related cells. GenJet? reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
90%
Cell culture conditions
DMEM with 4.5 g/L glucose, 10% FBS
Transfection Medium
DMEM with 4.5 g/L glucose, 1% FBS
GenJet? (μl) : DNA (μg) Ratio
3:1
Diluent for DNA and Transfection Reagent
Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet?/DNA Complex
15 minutes at RT
Presence of Serum/Antibiotics during Transfection
Yes in presence of 1% FBS
Change Medium 5 Hours After Transfection
No
Maximal Efficiency
48 hours
Transfection Results:
Reporter Gene
EGFP
Plasmid
pEGFP-N3 (CMV promoter)
Efficiency (GFP %)
85%
Storage Condition Store at 4 °C. If stored properly, the product is stable for 12 months or longer A Picture Showing Transfection Efficiency of GenJet? Reagent on Rin-5F Cells. Rin-5F cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet?. The efficiency was checked 24 hours post transfection
