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GenJet? In Vitro DNA Tranfection Reagent for WEHI-231 Cells

￥ 1,560.00

購買(mǎi)數量：

  • 目錄號: SL100489-WEHI
  • 包裝: 1.0 ml

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Description

GenJet? DNA In Vitro Tranfection Reagent for WEHI-231 is pre-optimized for transfecting WEHI-231 cells.? WEHI-231 is a murine B cell lymphoma that has been used extensively as a model for the immature stage B cell and its functional response to Ag receptor cross-linking as a model for immature B cell tolerance. This cell line expresses sIgM, CD5, and FcR gamma, but lacks the B cell- specific isoform of CD45 (B220).

Refer to the following optimal transfection conditions for maximal transfection efficiency on WEHI-231 cells. GenJet? reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:

Confluence on the day of transfection
 95%
Cell culture conditions DMEM with 4.5 g/L glucose, 10% FBS
GenJet? (μl) : DNA (μg) Ratio 3:1
Diluent for DNA and Transfection Reagent
 Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet?/DNA Complex 15 minutes at RT
Presence of Serum/Antibiotics during Transfection Yes
Change Medium After Transfection Yes, ~5 Hours After Transfection
Maximal Efficiency 48 hours

Transfection Results:

Reporter Gene
 EGFP
Plasmid
 pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 66%

Storage Condition
 Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet




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