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GenJet? In Vitro DNA Tranfection Reagent for 3LL Cells

￥ 1,560.00

購買(mǎi)數量：

  • 目錄號: SL100489-3LL
  • 包裝: 1.0 ml

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Description

GenJet? DNA In Vitro Tranfection Reagent for 3LL cell is pre-optimized for transfecting 3LL cells. 3LL also called Lewis lung carcinoma was discovered by Dr. Margaret R. Lewis of the Wistar Institute in 1951. This tumor originated spontaneously as a carcinoma of the lung of a C57BL mouse. The tumor does not appear to be grossly hemorrhagic and the majority of the tumor tissue is a semifirm homogeneous mass.

Refer to the following optimal transfection conditions for maximal transfection efficiency on 3LL cells. GenJet? reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:

Confluence on the day of transfection
 80%
Cell culture conditions DMEM with 4.5 g/L glucose, 10% FBS
GenJet? (μl) : DNA (μg) Ratio 3:1
Diluent for DNA and Transfection Reagent
 Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet?/DNA Complex 15 minutes at RT
Presence of Serum/Antibiotics during Transfection Yes
Change Medium After Transfection Yes, 18~24 Hours After Transfection
Maximal Efficiency 48 hours

Transfection Results:

Reporter Gene
 EGFP
Plasmid
 pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 88%

Storage Condition
 Store at 4 °C. If stored properly, the product is stable for 12 months or longer

A Picture Showing Transfection Efficiency of GenJet? Reagent on 3LL Cells. 3LL cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet?. The efficiency was checked 24 hours post transfection

Data Sheet




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