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GenJet? In Vitro DNA Tranfection Reagent for PC-12 Cell

￥ 1,560.00

購買(mǎi)數量：

  • 目錄號: SL100489-PC12
  • 包裝: 1.0 ml

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Description

GenJet? DNA In Vitro Tranfection Reagent for PC12 cell is pre-optimized for most efficiently transfecting PC12 cells. PC12 is a cell line derived from a pheochromocytoma of the rat adrenal medulla.? PC12 cells stop dividing and terminally differentiate when treated with nerve growth factor.? This makes PC12 cells useful as a model system for neuronal differentiation.

Refer to the following optimal transfection conditions for maximal transfection efficiency on PC12 cells. GenJet? reagent, 1.0 ml, is sufficient for 660 transfections in 24 well plates or 330 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:

Confluence on the day of transfection
Cell culture conditions
GenJet? (μl) : DNA (μg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet?/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid
Efficiency (GFP %)

  


~80%
DMEM with 4.5 g/L glucose, 10% FBS
3:1
Serum-free DMEM with 4.5 g/L glucose
15 minutes at RT
Yes
Yes, ~5 Hours After Transfection
48 hours

EGFP
pEGFP-N3 (CMV promoter)
50%

Storage Condition
 Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet




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